Analysis of phospholipids in microalga Nitzschia closterium by UPLC-Q-TOF-MS     

严小军  李海英  徐继林  周成旭 《中国海洋湖沼学报》2010,28(1):106-112

Precise structural identification of phospholipids in the microalga Nitzschia closterium has been established using ultra performance liquid chromatography-electrospray ionization-quadrupole-time of flight-mass spectrometry (UPLC-ESI-Q-TOF-MS) for direct analysis of total lipid extracts. Mass spectrometry was performed in reflective time-of-flight using electron spraying ionization in negative mode. Phospholipid molecular species identification was based on the characteristic product ions and neutral loss yielded by different phospholipids under ESI-MS/MS mode. The molecular species were confirmed by the carboxylate anions produced by phospholipids in negative mode; the regiospecificity of the two acyl chains was determined from the ratio of sn-1 to sn-2 carboxylate anion abundances. As a result, 18 lipid molecular species were identified for the first time in this microalga, comprising seven phosphatidylcholines (PC), two phosphatidylethanolamines (PE), two phosphatidylinositols (PI), and seven phosphatidylglycerols (PG). Lipid standards of PC, PE, PI, and PG were added to the total lipids as internal standards for semiquantitative analysis, revealing concentrations of phospholipids in this species between 0.09 and 3.37 nmol/mg. This method can produce a full structural profile of intact phospholipid molecular species and can be used for study of the physiological and ecological functions of lipids by monitoring their individual changes over time.  相似文献   

汶川8.0级地震的余震触发作用和对断层的应力加卸载作用   总被引：1，自引：0，他引：1  

解朝娣  朱元清  雷兴林  于海英 《地震地磁观测与研究》2010,31(2):12-18

采用可变滑动震源模型,计算和研究了汶川M_S 8.0地震产生的应力变化及对后续6次强余震的应力触发作用,并定量分析了汶川地震对附近活动断裂带的库仑应力加卸载作用。结果显示:汶川地震产生的库仑破裂应力变化分布图案复杂性强,主要由发震断裂复杂的空间展布形态以及震源断层活动具有逆冲兼右旋走滑两种性质决定。汶川地震产生的库仑破裂应力变化对多数强余震存在一定的触发作用。汶川地震的发生对附近的断裂带有不同程度的影响,其中青川断裂中段和北段、岷江断裂南段和灌县-江油断裂南段,主要是以强烈的库仑破裂应力加载作用占主导地位,有利于强余震序列的孕育、加速以至于发生;东昆仑断裂带和鲜水河断裂带受到了轻微的应力加载作用;龙泉山断裂带、华莹山断裂带和西秦岭北缘断裂带主要受到了轻微的卸载作用。  相似文献   

青海湖流域50年来(1956-2007年)河川径流量变化趋势——以布哈河和沙柳河为例   总被引：1，自引：0，他引：1  

李岳坦  李小雁  崔步礼  彭海英  伊万娟 《湖泊科学》2010,22(5):757-766

通过对青海湖流域布哈河和沙柳河50年来的河川径流量分析发现,布哈河和沙柳河年径流量50年来没有显著的变化趋势,这两条河流的河川径流量对青海湖水位下降所起的作用不明显;布哈河月平均流量的年际变化在1月、2月和3月有减少的趋势,沙柳河月平均流量的年际变化在1月、2月、4月和5月亦有减少的趋势;布哈河的径流量大于沙柳河的径流量,在55%-91%频率范围内,布哈河的径流量小于沙柳河的径流量,在其它频率范围内,布哈河的径流量显著大于沙柳河的径流量,在一年中,布哈河和沙柳河的月径流量具有显著的差异;布哈河来水丰沛期是20世纪60年代,贫乏期是90年代,70和80年代为平水期;沙柳河月径流量从20世纪60年代到90年代一直比较稳定,没有发生显著的变化.  相似文献   

盆地深部穹隆构造发育特征、成因机制及石油地质意义——以塔里木盆地顺北地区为例   总被引：1，自引：0，他引：1  

高天  邓尚  李海英  刘雨晴  黄诚  艾合买提江·阿不都热和曼 《地质论评》2022,68(1):217-232

塔里木盆地顺北地区奥陶系碳酸盐岩中广泛发育穹隆构造.为研究盆地深部穹隆构造的发育特征、成因机制及石油地质意义,基于顺北地区高精度三维地震资料,对穹隆构造开展几何学定量解析,确定其空间分布规律及内部结构样式;结合区域构造背景及盆内构造发育特征,明确其成因机制;在此基础上,结合生产动态数据探讨穹隆构造的控储—控藏作用.研究...  相似文献   

Carbon accumulation, nitrogen and phosphorus use efficiency of Sophora davidii seedlings in response to nitrogen supply and water stress   总被引：1，自引：0，他引：1  

F.Z. Wu  W.K. Bao  Z.Q. Zhou  N. Wu 《Journal of Arid Environments》2009,73(12):1067-1073

A greenhouse experiment was conducted to explore whether additional nitrogen (N) supply could enhance carbon (C) accumulation, and phosphorus (P) use efficiency (NUE_P) of Sophora davidii seedlings under dry conditions. Two-month-old seedlings were subjected to a completely randomized design with three water (80, 40 and 20% water field capacity (FC)) and three N supply (N0: 0, Nl: 92 and Nh: 184 mg N kg⁻¹ soil) regimes. Water stress decreased C, N and P accumulation, NUE_P, N and P uptake efficiency (NUtE_N and NUtE_P) regardless of N supply. The S. davidii seedlings exhibited strong responses to N supply, but the responses were not consistent with the various N supply levels. Nl increased C, N and P accumulation, and improved NUE_P, NUtE_N and NUtE_P in the same water treatment. In contrast, Nh did few or even depress effects on C, N and P accumulation, and NUE_P, although NUtE_N and NUtE_P increased with Nh in the same water treatment. Even so, NUE_N decreased with increase of N supply in the same water treatment. The results suggested that appropriate or low N supply should be recommended for S. davidii seedling establishment in dry environment by improving C accumulation and NUE_P.  相似文献   

Characteristics and cause analysis of heavy haze in Changchun City in Northeast China   总被引：1，自引：1，他引：0  

Siqi Ma  Weiwei Chen  Shichun Zhang  Quansong Tong  Qiuyang Bao  Zongting Gao 《中国地理科学(英文版)》2017,27(6):989-1002

Northeast China has been reported as having serious air pollution in China with increasing occurrences of severe haze episodes. Changchun City, as the center of Northeast China, has longstanding industry and is an important agricultural base. Additionally, Changchun City has a long winter requiring heating of buildings emitting pollution into the air. These factors contribute to the complexity of haze pollution in this area. In order to analyze the causes of heavy haze, surface air quality has been monitored from 2013 to 2015. By using satellite and meteorological data, atmospheric pollution status, spatio-temporal variations and formation have been analyzed. Results indicated that the air quality in 88.9% of days exceeding air quality index(AQI) level-1 standard(AQI 50) according to the National Ambient Air Quality Standard(NAAQS) of China. Conversely, 33.7% of the days showed a higher level with AQI 100. Extreme haze events(AQI 300) occurred frequently during agricultural harvesting period(from October 10 to November 10), intensive winter heating period(from Late-December to February) and period of spring windblown dust(April and May). Most daily concentrations of gaseous pollutants, i.e., NO_2(43.8 μg/m~3), CO(0.9 mg/m~3), SO_2(37.9 μg/m~3), and O_3(74.9 μg/m~3) were evaluated within level-1 concentration limits of NAAQS standards. However, particulate matter(PM_(2.5) and PM10) concentrations(67.3 μg/m~3 and 115.2 μg/m~3, respectively) were significantly higher than their level-1 limits. Severe haze in spring was caused by offsite transported dust and windblown surface soil. Heavy haze periods during fall and winter were mainly formed by intensive emissions of atmospheric pollutants and steady weather conditions(i.e., low wind speed and inversion layer). The overlay emissions of widespread straw burning and coal combustion for heating were the dominant factors contributing to haze in autumn, while intensive coal burning during the coldest time was the primary component of total emissions. In addition, general emissions including automobile exhaust, road and construction dust, residential and industrial activities, have significantly increased in recent years, making heavy haze a more frequent occurrence. Therefore, both improved technological strategies and optimized pollution management on a regional scale are necessary to minimize emissions in specified seasons in Changchun City, as well as comprehensive control measures in Northeast China.  相似文献   

水文气象研究进展   总被引：5，自引：0，他引：5  

赵琳娜  包红军  田付友  梁莉  刘莹 《气象》2012,38(2):147-154

从面向流域的定量降水估测与预报、流域水文模型、水文气象耦合预报三个方面系统介绍水文气象研究进展。研究指出,融合天气雷达、卫星遥感及实况降水等多源信息是精细化定量降水估测产品的主要发展方向;采用多模式降水预报集成技术是提高定量降水预报精度的重要途径;分布式水文模型是流域水文模型的发展方向;引入定量降水预报的水文气象耦合预报模式可以延长洪水预报预见期,水文集合预报是水文预报方法的有效解决途径,而数值预报模式与水文模型的双向耦合模式是另一重要发展方向。  相似文献   

乌兰察布地区一次大范围沙尘天气过程分析     

刘建梅  高晶  包福祥 《内蒙古气象》2012,(2):3-4,10

运用常规气象观测资料,从天气环流形势的演变过程及有关物理量变化状况,对2010年3月19—20日发生在乌兰察布地区沙尘天气过程做了客观分析。分析结果表明：造成这次沙尘天气过程的主要影响天气系统是蒙古气旋和地面冷锋。蒙古气旋后部西北强冷空气侵入为起源于蒙古国西南部以及内蒙古西中部周边沙尘的输送提供了动力条件;地面冷锋过境使该地区温度梯度增大,空气对流上升加剧,为上游输送而来的尘土、沙石卷入空中浮悬提供了抬升条件。两者共同作用促使沙尘天气过程持续时间长、范围广,并伴有扬沙和沙尘暴出现。  相似文献   

内蒙古极端低温事件发生的环流特征及预测概念模型     

司瑶冰  李海英  杨晶  杨丽桃  刘秀荣 《内蒙古气象》2012,(6):3-9

利用50a（1961-2010年）的日最低气温计算阈值,超过此阈值为极端低温事件。将内蒙古东、中、西部的极端最低气温事件进行四季划分统计．讨论分析这些地区的极端低温事件与大气环流、海洋因子的关系。分析研究表明：内蒙古极端低温事件主要发生在冬季（12月至次年2月）,集中在20世纪60年代初至80年代中期,80年代中期至90年代末基本上没有发生,90年代末以后又陆续发生,但强度、范围明显减小。极端低温事件与北半球极涡、北极涛动、北太平洋涛动、南方涛动、海气相互作用、副热带高压、两风带环流存在着大体一致的年代际震荡趋势,同时与冷空气南下的路径有关,而且相关关系显著。通过相关检验,建立内蒙古极端低温事件的预测概念模型．以此应J}=fj于实际的业务当中．减少极端事件发生引发的各方面损失。  相似文献   

采用SilviScan-3TM研究木材性质的方法及其在气候变化研究中的应用     

徐金梅  吕建雄  鲍甫成  肖军  Robert Evans  黄荣凤  徐明 《气候变化研究进展》2012,8(2):124-130

系统介绍了利用SilviScan-3TM测量细胞结构、木材密度、微纤丝角和划分年轮界线的方法,并以祁连山青海云杉为例,分析青海云杉6个木材性质参数(年轮细胞直径、年轮细胞壁厚、年轮宽度、年轮密度、年轮微纤丝角、年轮弹性模量)与气候因子的关系,以期为利用多个树轮参数研究气候提供参考。结果表明:6个木材性质参数与月平均气温和月降水量都有显著相关的月份,但显著相关的时间段不同,并且微纤丝角和细胞结构参数中包含的气候信息强于常用的年轮宽度和年轮密度。SilviScan-3TM测量木材性质参数的优越性体现在:测量精度高、速度快,能在同一个试样上测量多个参数并能精确定年。  相似文献